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Sampling and bias Why unbiasedness is desirable Sources of bias in microscopy Sampling bias Systematic bias The hierarchical nature of microscopical investigations Stereology-geometrical quantification in 3D Think in three dimensions-a macroscopic analogy by thought experiment Points probe volume Lines probe surface Planes probe length Volumes probe number Dimensions and sectioning Geometrical probes Ratios and densities The reference space Image provided by Dr Gesa Staats de Yanes. Q175.H86 2004 5022.822–dc22 2004015841 Production Editor: Catherine Jones Pseudocolored image of neurons from Layer II of rat neocortex.
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cm.-(Advanced methods) Includes bibliographical references and index. Unbiased stereology/C.V.Howard, M.Reed.
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Distributed in the USA by Fulfilment Center Taylor & Francis 10650 Toebben Drive Independence, KY 41051, USA Toll Free Tel: +1 8 E-mail: Distributed in Canada by Taylor & Francis 74 Rolark Drive Scarborough, Ontario MIR 4G2, Canada Toll Free Tel.: +1 8 E-mail: Distributed in the rest of the world by Thomson Publishing Services Cheriton House North Way Andover, Hampshire SP10 5BE, UK Tel.: +44 (0)1264 332424 E-mail: Library of Congress Cataloging-in-Publication Data Howard, Vyvyan. ISBN 1 85996 0898 (Print Edition) Garland Science/BIOS Scientific Publishers 4 Park Square, Milton Park, Abingdon, Oxon, OX14 4RN, UK and 270 Madison Avenue, New York, NY 10016, USA World Wide Web home page: Garland Science/BIOS Scientific Publishers is a member of the Taylor & Francis Group. A CIP catalogue record for this book is available from the British Library. No part of this book may be reprinted or reproduced or utilized in any form or by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying and recording, or in any information storage or retrieval system, without permission in writing from the publishers. “To purchase your own copy of this or any of Taylor & Francis or Routledge’s collection of thousands of eBooks please go to © Garland Science/BIOS Scientific Publishers, 2005 First edition published 1998 Second edition published 2005 All rights reserved. This edition published in the Taylor & Francis e-Library, 2008. Unbiased Stereology Three-Dimensional Measurement in Microscopy Second EditionĬ.V.Howard & M.Reed Developmental Toxico-Pathology Research Group, Department of Human Anatomy and Cell Biology, University of Liverpool, Liverpool, UK Preface to the second edition.Ģ Random sampling and random geometry.ģ Estimation of reference volume using the Cavalieri method.Ĥ Estimation of component volume and volume fraction.Ħ Estimation of total surface area and surface density.Ĩ Stereological analysis of layered structures.ġ0 Statistics for stereologists.ġ1 Single-object stereology.Īppendix A: Practical gadgets for stereology.Īppendix B: Set of stereological grids.Īppendix C: Worked answers to exercises.Īppendix D: Useful addresses.